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KMID : 0644120090220030117
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Journal of Oriental Gynecology
2009 Volume.22 No. 3 p.117 ~ p.134
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Effects of Scutellariae Radix on Gene Expression of Human Cervical Cancer Cells(SNU-703)
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Jo Hyun-Jung
Gu Hee-Jun
Cho Seong-Hee
Park Kyoung-Mi
Yang Seung-Jeong
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Abstract
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Purpose: In the theory of traditional medicine, Scutellariae Radix (SR) can clear away heat and remove dampness, purge the sthenic fire and remove toxic materials, cool blood and stop bleeding to prevent miscarriage. Recently, SR is known to have anti-cancer activity. For this reason, the present author designed to investigate the effect of SR on proliferation rates of cervical cancer cell line, then effects on genetic profile by SR.
Methods: The genetic profile for the effect of SR on human derived cervical cancer cell line, SNU-703, was measured using microarray technique, and the functional analysis on these genes was conducted.
Results: Total 519 genes were up-regulated and 606 genes down-regulated in cells treated with SR. Genes induced or suppressed by SR were all mainly concerned with metabolic process, regulation of biological process and protein binding. The network of total protein interactions was measured using cytoscape program, and some key molecules, such as TNFRSF1A, AKT1, MAPK3, and STAT3 that can be used for elucidation of therapeutical mechanism of medicine in future were identified.
Conclusion: These results suggest possibility of SR as anti-cancer drug and also suggest that related mechanisms are involved in TNFRSF1A, AKT1, MAPK3, and STAT3 related signalling pathways.
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KEYWORD
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Cervical cancer, Scutellariae Radix, Gene expression, Anticancer drug, Microarray
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